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目的:观察转化生长因子(TGF)-β、白细胞介素(IL)-6和IL-23对柯萨奇病毒性心肌炎(VMC)小鼠脾中Th17细胞分化增殖的影响。方法:以6周龄雄性Balb/c小鼠腹腔注射柯萨奇病毒液建立VMC模型,1周后磁珠分离脾脏CD4+T淋巴细胞,分成对照组、TGF-β+IL-6组和TGF-β+IL-6+IL-23组进行体外培养。5d后应用流式细胞术测定刺激后Th17细胞的百分比;逆转录-聚合酶链式反应测定细胞中IL-17mRNA的表达;ELISA测定细胞培养上清液IL-17的水平。结果:与对照组比较,TGF-β+IL-6组Th17细胞数稍增加[(1.64±0.43)%∶(1.96±0.35)%],IL-17mRNA表达水平及细胞培养上清液IL-17浓度稍增加,但差异无统计学意义(P>0.05)。而TGF-β+IL-6+IL-23组Th17细胞数显著增加(4.08±0.77)%、IL-17mRNA表达水平及细胞培养上清液IL-17浓度均明显高于前2组,差异有统计学意义(P<0.05)。结论:外源性TGF-β联合IL-6对VMC小鼠脾中已分化的Th17细胞无增殖作用,而添加外源性IL-23则能显著增加Th17细胞数及其效应分子IL-17的表达。
Objective: To observe the effects of transforming growth factor (TGF) -β, interleukin (IL) -6 and IL-23 on the proliferation and differentiation of Th17 cells in the spleen of mice with coxsackievirus myocarditis (VMC). Methods: The VMC model was established by intraperitoneal injection of Coxsackievirus in 6-week-old male Balb / c mice. One week later, splenic CD4 + T lymphocytes were isolated by magnetic beads and divided into control group, TGF-β + IL-6 group and TGF -β + IL-6 + IL-23 group were cultured in vitro. After 5 days, the percentage of Th17 cells was measured by flow cytometry. The expression of IL-17 mRNA in the cells was detected by reverse transcription-polymerase chain reaction. The level of IL-17 in the cell culture supernatant was measured by ELISA. Results: Compared with the control group, the number of Th17 cells in TGF-β + IL-6 group was slightly increased (1.64 ± 0.43% vs (1.96 ± 0.35)%, IL-17 mRNA and IL-17 Concentration increased slightly, but the difference was not statistically significant (P> 0.05). The number of Th17 cells in TGF-β + IL-6 + IL-23 group was significantly increased (4.08 ± 0.77)%, IL-17 mRNA expression and cell culture supernatant IL-17 concentrations were significantly higher than the previous two groups, the difference was Statistical significance (P <0.05). CONCLUSION: Exogenous TGF-β combined with IL-6 has no proliferative effect on differentiated Th17 cells in spleen of VMC mice, whereas addition of exogenous IL-23 can significantly increase the number of Th17 cells and its effector molecule IL-17 expression.