目的　研究鸡 Tie- 2受体胞外片段 (配体结合域 )的原核表达以及目的蛋白对小鼠的抗原性。方法　用 PCR方法从既往构建的包含鸡 Tie- 2受体胞外段基因的表达质粒扩增目的片段 ,进而构建 PQE原核表达载体并诱导其目的蛋白的表达 ,将目的蛋白进一步纯化、复性并免疫小鼠 ,获得抗血清 ,用 EL ISA和 Western blotting检测抗血清中抗体的存在。结果　经酶切分析及测序鉴定表明获得了鸡 Tie- 2受体胞外目的片段的 PQE原核表达载体 ,能高效表达目的蛋白 ,将其免疫小鼠可产生抗重组蛋白的抗体。结论　用原核表达的方式可成功的获得鸡Tie- 2受体胞外目的片段的蛋白 ,并对小鼠产生免疫原性 Objective To study the prokaryotic expression of the extracellular fragment of Tie-2 receptor (ligand-binding domain) and the antigenicity of the target protein in mice. Methods The target fragment was amplified from a previously constructed expression plasmid containing the extracellular domain of chicken Tie-2 receptor by PCR, and then the prokaryotic expression vector of PQE was constructed and the expression of the target protein was induced. The target protein was further purified and renatured The mice were immunized to obtain antiserum, and the presence of antibodies in the antisera was detected by ELISA and Western blotting. Results The results of enzyme digestion analysis and sequencing showed that prokaryotic expression vector of PQE gene fragment of chicken Tie-2 receptor was obtained, which could express the target protein efficiently and could immunize mice to produce anti-recombinant protein antibody. Conclusion The prokaryotic expression system can successfully obtain the extracellular fragment of chicken Tie-2 receptor protein, and immunogenicity in mice