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目的:建立独藤丸质量标控制方法,为进一步扩大应用提供参考。方法:采用薄层色谱法(TLC)对独藤丸中独活、桑寄生、茯苓、甘草药材进行定性鉴别;采用高效液相色谱法(HPLC)测定独藤丸中蛇床子素的含量。结果:TLC定性鉴别斑点清晰,分离度好,色谱特征明显;HPLC测定蛇床子素20～200 mg.mL-1呈良好的线性关系(r=0.999 7,n=5),平均回收率为99.57%,RSD 2.309%。结论:方法简便、可靠、专属性强、重复性好、方法准确,可用于独藤丸的质量控制。
Objective: To establish the quality control method of Danpu Pill and provide reference for further application. Methods: TLC, TLC and HPLC were used to qualitatively identify the independent living, the parasiticus, the tuckahoe and the licorice root in the single-rattan pill. The content of the Osthole in alone rattan pill was determined by high performance liquid chromatography (HPLC). Results: TLC qualitative identification spots were clear, the separation was good, and the chromatographic characteristics were obvious. The HPLC determination of osthole 20 ~ 200 mg.mL-1 showed a good linear relationship (r = 0.999 7, n = 5) with an average recovery of 99.57 %, RSD 2.309%. Conclusion: The method is simple, reliable, specific, reproducible and accurate. It can be used for the quality control of Dan Fuju Pills.