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本文用扫描电子显微技术结合 DNS法测定卵磷脂-纤维素酶-水(PC-Cel-H2O)体系中纤维素酶三组分(内切葡聚糖酶,外切葡聚糖酶,β-葡萄糖苷酶)的活性,分析体系中各组分间相互作用,并由此建立起一个模型,用于描述 PC-Cel-H2O 中纤维素酶的作用机理:当 Cpc 很小时,极性头部作用于内切葡聚糖酶,使其构象变化,并通过后者抑制了另二种酶的活性;当增大到一定浓度(当Ccel为0.13m g/m l时,此 Cpc 为0.3m g/m l)时,PC 的作用使内切葡聚糖酶完全失活,并开始以非极性尾部作用于外切葡聚糖酶;当大于PC 的临界胶束浓度时,外切酶随非极性部位进入胶束或脂双层而失活,内切葡聚糖酶却因PC对其作用的变小而部分恢复活性,β-葡萄糖苷酶在后两种情况下均有活性
In this paper, scanning electron microscopy combined with DNS method for the determination of lecithin - cellulase - water (PC-Cel-H2O) system of three components of cellulase (endoglucanase, exoglucanase, β - glucosidase) activity, the interaction between the various components of the system was analyzed, and a model was established to describe the mechanism of action of cellulase in PC-Cel-H2O: When Cpc is small, the polarity of the head Part acts on the endoglucanase to conformational change and inhibits the activity of the other two enzymes through the latter; when it increases to a certain concentration (when Ccel is 0.13 m g / ml, this Cpc is 0.3 m g / m l), the action of PC completely inactivated the endoglucanase and began to act on the exoglucanase with a nonpolar tail; when it is larger than the critical micelle concentration of PC, Exo-enzyme enters the micellar or lipid bilayer with the non-polar site inactivation, endoglucanase partially due to the role of PC to restore activity, β-glucosidase in the latter two cases Are active