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目的检测新生儿胰岛素样生长因子Ⅰ(IGF-Ⅰ)启动子区域(CA)n重复标记位点的基因多态性,及新生儿血清IGF-Ⅰ浓度,探讨基因多态性与血清IGF-Ⅰ浓度的关系,分析相关的遗传背景。方法202名健康新生儿根据胎龄分为早产(111名)和足月(91名)两组,记录出生体重、身长;于生后第3～5天取血,酶联免疫法检测血清IGF-Ⅰ、生长激素(GH)浓度;提取DNA,分析IGF-Ⅰ启动子区域基因多态性。结果在202名新生儿中,发现7种不同的等位基因及20种基因型。7种等位基因频率分别为7.92%、14.60%、43.56%、7.43%、21.78%、3.96%、1.10%,其中携带190等位基因的早产新生儿的血清IGF-Ⅰ水平及出生体重相对较低。未发现基因多态性与出生身长相关。结论IGF-Ⅰ基因启动子区域190等位基因可能与早产新生儿出生体重及血清IGF-Ⅰ浓度相关。本研究提示基因可能影响出生前生长和血清IGF-Ⅰ水平的关系。
Objective To detect the genetic polymorphism of neonatal insulin-like growth factor Ⅰ (CA) n repeat marker loci and serum IGF-Ⅰ concentration in neonates to investigate the relationship between gene polymorphism and serum IGF-Ⅰ Concentration of the relationship between the analysis of the genetic background. Methods Totally 202 healthy newborns were divided into preterm (111) and full term (91) groups according to their gestational age. The birth weight and length were recorded. Blood samples were collected on the 3rd to 5th days after birth and the serum IGF Ⅰ, growth hormone (GH) concentration; DNA extraction, analysis of IGF-Ⅰ promoter region gene polymorphism. Results In 202 newborns, seven different alleles and 20 genotypes were found. The frequencies of the seven alleles were 7.92%, 14.60%, 43.56%, 7.43%, 21.78%, 3.96% and 1.10%, respectively. The serum IGF-Ⅰ level and birth weight of premature neonates with 190 alleles low. No genetic polymorphism was found to be related to birth length. Conclusion The 190 allele of IGF-Ⅰ gene promoter region may be related to the birth weight and serum IGF-Ⅰ concentration of newborn infants. This study suggests that the gene may affect the relationship between prenatal growth and serum IGF-I levels.