论文部分内容阅读
Objective: To investigate the anti-inflammatory effect of erythropoietin (EPO) pretreatment on cardiomyocytes ex-posed to hypoxia/reoxygenation injury (H/R) and explore the possible mechanism. Methods: The cultured neonatal rats ventricular cardiomyocytes were divided randomly into 4 groups, con-trol group (C group), EPO pretreatment group (E group), EPO and pyrrolidine dithiocarbamate (PDTC) pretreatment group (EP group) and PDTC pretreatment group (P group). After 24 hours pretreatment, the cardiomyocytes were exposed to H/R. After pretreatment and H/R, the expression of tumor necrosis factor- α (TNF- α ) gene in all the groups was detected by RT-PCR and Western blot. The nuclear factor- KB (NF- kB) activity was detected by electrophoretic mobility shift assay (EMSA) and the inhibitor-kBα (I- kBα)protein level was detected by Western blot. Results: The decrement of I- K B α protein and the in-creasing NF- kB activity were found in cardiomyocytes pre-treated with EPO before H/R compared to other groups (t=-3.321,4.183, P