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目的:了解白细胞介素-6(IL-6)基因-572C/G多态性对冠心病(CHD)发病易感性及血清IL-6水平的影响。方法:应用聚合酶链反应-限制性片段长度多态性分析方法,测定245例CHD患者(CHD组)和260例正常对照者(对照组)的IL-6基因-572C/G多态性,探讨其与CHD的相关关系;并应用酶联免疫吸附试验法检测研究人群血清IL-6水平,观察基因型对血清IL-6水平的影响。结果:IL-6基因-572C/G基因型和等位基因频率在2组间分布差异有统计学意义,CHD组GG基因型和G等位基因频率均显著高于对照组(P<0.01);相对于CC基因型,暴露于CG、GG基因型的相对危险度分别为1.46(95%CI:1.01~2.10,P<0.05)和5.19(95%CI:1.69~15.89,P<0.01);不同基因型患者间血清IL-6水平差异无统计学意义(P>0.05)。结论:IL-6基因-572C/G多态性可能是中国汉族人群CHD发病的遗传危险因素之一,但该多态性对血清IL-6水平无影响。
Objective: To investigate the effect of interleukin-6 (IL-6) -572C / G polymorphism on susceptibility to coronary heart disease (CHD) and serum IL-6 level. Methods: The -572C / G polymorphism of IL-6 gene in 245 CHD patients (CHD group) and 260 normal controls (control group) was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) To explore its relationship with CHD; and serum IL-6 levels were measured by enzyme-linked immunosorbent assay to observe the impact of genotype on serum IL-6 levels. Results: There were significant differences in the distribution of -572C / G genotype and allele between IL-6 gene and allele in the two groups. The frequencies of GG genotype and G allele in CHD group were significantly higher than those in control group (P <0.01) The relative risk of exposure to CG and GG genotypes was 1.46 (95% CI: 1.01-2.10, P <0.05) and 5.19 (95% CI: 1.69-15.89, P <0.01) There was no significant difference in serum IL-6 levels between different genotypes (P> 0.05). CONCLUSION: The -572C / G polymorphism of IL-6 gene may be one of the genetic risk factors for CHD in Chinese Han population. However, this polymorphism has no effect on serum IL-6 level.