目的:研究芪菖治瘫口服液是否对脑缺血再灌注损伤大鼠脑组织一氧化氮和一氧化氮合成酶(nitricoxidesynthase,NOS)产生影响,为研究开发中药新药提供理论基础。方法:用三动脉夹闭法造成大鼠脑缺血模型动物,同时用芪菖治瘫口服液、补阳还五汤、维生素E灌胃给药7d,1次/d。分为手术对照组、模型对照组、芪菖治瘫口服液高剂量组、低剂量组、补阳还五汤组及维生素E组。检测大鼠脑组织一氧化氮和NOS含量。结果:模型对照组大鼠脑组织一氧化氮(17.27±2.11)μmol/g与NOS含量(0.13±0.03)μmol明显低于手术对照组犤(44.72±7.72)μmol/g(0.27±0.05)μmol,P<0.01犦;各给药组大鼠脑一氧化氮及NOS含量均不同程度低于手术对照组,而高于模型对照组,且有统计学差异。结论:芪菖治瘫口服液可明显增加脑内一氧化氮和NOS含量,改善脑组织内环境,进而保护脑组织和治疗脑梗死。 Objective: To investigate whether Qizhizhi Oral Liquid has effects on nitric oxide and nitric oxide synthase (NOS) in brain tissue of rats with cerebral ischemia-reperfusion injury, and provides a theoretical basis for the research and development of new Chinese medicines. METHODS: Rat models of cerebral ischemia were established by three-artery clamping method. At the same time, Qizhizhiye Oral Liquid, Buyang Huanwu Decoction and Vitamin E were intragastrically administered for 7 days, once a day. They were divided into surgical control group, model control group, high dose group of Qizhizhi oral liquid, low dose group, Buyang Huanwu Decoction group and vitamin E group. Nitric oxide and NOS levels in brain tissue of rats were measured. Results: The levels of nitric oxide (17.27±2.11) μmol/g and NOS (0.13±0.03) μmol were significantly lower in the model control group than in the control group (44.72±7.72) μmol/g (0.27±0.05) μmol. , P <0.01 犦; Nitric oxide and NOS contents in the brains of rats in each treatment group were lower than the surgical control group, but higher than the model control group, and there were statistically significant differences. Conclusion: Qizhizhi Oral Liquid can significantly increase the content of nitric oxide and NOS in the brain, improve the internal environment of the brain, and then protect the brain tissue and treat cerebral infarction.