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应用AFLP技术对赤点石斑鱼Epinephelus akaara7个地理群体进行了遗传多样性及遗传分化的分析。结果显示:不同群体的遗传多样性差异较大,大亚湾群体和舟山群体遗传多样性最低,湛江群体最高;通过UPGMA聚类,7个群体88个个体明显分成3支,三亚群体单独聚为一支(Clade A),湛江群体的部分个体聚类为一支(Clade B),湛江群体剩余个体和其他5个群体的个体聚为一支(Clade C)。其中在分支C中存6个小的分支,这6支中个体间基本以地理群体进行聚类。研究结果为赤点石斑鱼种质资源保护和遗传改良提供了遗传学依据。
AFLP technique was used to analyze the genetic diversity and genetic differentiation of seven geographical populations of Epinephelus akaara. The results showed that the genetic diversity of different groups was quite different, the genetic diversity of Daya Bay and Zhoushan was the lowest, and the highest in Zhanjiang. By UPGMA clustering, 88 individuals of 7 populations were apparently divided into 3 branches and Sanya subpopulations were clustered together (Clade A), a part of Zhanjiang group (Clade B), the rest of Zhanjiang group and the other five groups (Clade C). Among them, there are 6 small branches in the branch C, and these 6 individuals are basically clustered by geographical groups. The results provide a genetic basis for the conservation and genetic improvement of Red Grouper germplasm resources.