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活血化瘀是治疗恶性肿瘤的有效方法之一。本文观察了血瘀型恶性肿瘤病人的红细胞膜流动性、微粘度及血液流变性等七项指标,对血瘀型恶性肿瘤的本质作了初步探讨。 材料与方法 一、红细胞膜流动性测定 红细胞悬液的制备:取静脉血2ml,肝素抗凝,离心后用磷酸缓冲液(PBS)洗三次,以高倍显微镜下找不到血小板为度。配成10~(10)个红细胞/ml的悬液备用。使用时用pH7.4的PBS稀释成2×10~(-6)mol/L中的DPH。荧光偏振度测量:于3ml×10~(-6)mol/L的DPH液中加
Blood circulation is one of the effective ways to treat malignant tumors. This article observed seven patients with blood stasis type of malignant tumor of erythrocyte membrane fluidity, microviscosity and hemorheology and other seven indicators, the nature of blood stasis type of malignant tumor was initially explored. Materials and methods First, the determination of erythrocyte membrane fluid Preparation of erythrocyte suspension: venous blood 2ml, anticoagulant heparin, three times with phosphate buffered saline (PBS) washed three times, under high magnification microscope can not find the degree of platelets. Dubbed 10 ~ (10) red blood cells / ml of the suspension. When used with PBS pH7.4 diluted to 2 × 10 ~ (-6) mol / L of DPH. Fluorescence polarization measurement: in 3ml × 10 ~ (-6) mol / L of DPH solution plus