论文部分内容阅读
目的通过补硒对正常鸡胚脑和肝脏中谷胱甘肽过氧化物酶(GPx)和脱碘酶活性的影响,探讨补硒对于正常动物的影响。方法对孵育8d的鸡胚注射不同剂量的甲基硒代半胱氨酸(MSeC),含硒量分别为0、1、5、10、20μg,第20天时测定脑组织和肝脏中硒水平、GPx活性及脱碘酶活性。结果对正常鸡胚补硒,可有效提高脑和肝脏的硒水平及GPx活性,还可提高肝脏Ⅰ型脱碘酶(IDⅠ)活性,但对脑中Ⅱ型脱碘酶(IDⅡ)活性没有影响。补硒量与除脑IDⅡ活性外的其他指标都显著相关(P<0.01)。结论MSeC是一个有效的补硒制剂,对于正常鸡胚,补硒可提高肝脏、脑组织硒水平和肝GPx、IDⅠ活性及脑GPx活性,其补硒效应有随补硒量增加而增强的趋势。
Objective To investigate the effects of selenium supplementation on normal animals by the effect of selenium supplementation on the activity of glutathione peroxidase (GPx) and deiodinase in normal chicken embryo brain and liver. Methods Different concentrations of methyl selenocysteine (MSeC) were injected into chicken embryos incubated for 8 days. The selenium contents were 0, 1, 5, 10 and 20 μg respectively. The selenium levels in brain tissue and liver were measured on the 20th day. GPx activity and deiodinase activity. Results Supplementation of selenium in normal chicken embryos could increase selenium level and GPx activity in brain and liver, and also increase the activity of type Ⅰ deiodinase (IDⅠ) in liver, but had no effect on the activity of type Ⅱ deiodinase (IDⅡ) in brain . The amount of selenium was significantly correlated with other indexes except brain IDⅡactivity (P <0.01). Conclusion MSeC is an effective preparation of selenium supplementation. Supplementation of selenium can increase selenium level, hepatic GPx, IDⅠ activity and brain GPx activity of normal chick embryo. The effect of selenium supplementation increases with selenium supplementation .