通常,在细胞免疫组化技术中,待染色的细胞需要附着于固相介质以便下一步的处理。悬浮细胞通常生长在细胞离心涂片器上,并进一步用化学连接法固定在固相介质上,而贴壁细胞可以在细胞爬片、显微镜载玻片或透光性塑料支架上生长~([1])。在日常工作中,最常用方法是细胞爬片。免疫组化检测只能对单张细胞爬片逐片进行染色操作,故一般只能对单样本做一种抗体的检测。传统方法难以胜任大规模、多样本及多组别的医学实验科学研究,本文现提出一种,可进行大规模、多样本多组别的高效细胞爬片免疫组化方 Often, in cellular immunohistochemistry, the cells to be stained need to be attached to a solid-phase medium for further processing. Suspension cells are usually grown on a cytospin and further chemically immobilized on a solid phase medium while adherent cells can grow on a cell slide, a microscope slide, or a translucent plastic stent. 1]). In daily work, the most common method is cell climbing. Immunohistochemical detection can only be carried out on a single piece of cell slide staining operation, it is generally only a single sample to do an antibody test. The traditional method is not competent for large-scale, multi-sample and multi-group medical experimental scientific research, this paper presents a can be large-scale, multi-sample multiple groups of efficient cell cloning immunohistochemistry