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目的探讨人工合成单体抗氧化剂氨基胍(2-AG)对人胚肺二倍体成纤维细胞(2BS)复制性衰老的影响及其分子机制。方法观察2-AG 对2BS 细胞衰老表型、细胞代龄、传代速度、细胞周期、细胞增殖能力、晚期糖基化终末产物(AGEs)水平及衰老相关β半乳糖苷酶(SA-β-GaD阳性率的影响。结果 2-AG 可维持细胞的非衰老表型,增加2BS 细胞代龄19～20代,MTT 法分析发现用2-AG 孵育细胞84h,细胞增殖能力较对照组升高36%～40%。2-AG 显著加快了细胞的增殖速度,其培养细胞 S 期的比例较对照细胞升高约1倍。另外,2-AG 连续培养的细胞在老龄阶段 AGEs、SA-β-Gal 阳性率均显著低于老年对照细胞,而与年轻2BS 细胞相似。结论 2-AG 可有效延缓2BS细胞的复制性衰老。
OBJECTIVE: To investigate the effect and mechanism of synthetic monomeric anti-oxidant aminoguanidine (2-AG) on replicative senescence of human embryo lung diploid fibroblasts (2BS). Methods The effects of 2-AG on aging phenotype, cell age, passage velocity, cell cycle, cell proliferation, advanced glycation end products (AGEs) and aging related β-galactosidase (SA- GaD positive rate.Results 2-AG could maintain the non-aging phenotype of the cells and increase the age of 2BS cells from 19 to 20 generations. The results of MTT assay showed that the cell proliferation ability of 2-AG was increased by 36 % ~ 40% .2-AG significantly accelerated the rate of cell proliferation, the proportion of S cells in cultured cells increased by about 1 time compared with control cells.In addition, 2-AG continuously cultured cells in aged AGEs, SA- Gal positive rate was significantly lower than the old control cells, and similar to the young 2BS cells.Conclusion 2-AG can effectively delay the replication of 2BS cells aging.