论文部分内容阅读
目的:优选超临界CO2提取皱皮木瓜中齐墩果酸的工艺条件。方法:HPLC测定齐墩果酸含量,以齐墩果酸得率及含量的平均值为指标,应用单因素和正交试验考察提取温度、提取压力、分离温度、分离压力及携带剂种类5个影响因素,并将优选工艺与文献工艺进行对比分析。结果:超临界CO2提取齐墩果酸的最佳工艺条件为提取温度45℃,提取压力40 MPa,分离温度30℃,分离压力8 MPa,携带剂95%乙醇;超临界CO2提取优于其他方法。结论:该优选工艺稳定可行,可用于齐墩果酸的推广应用。
Objective: To optimize supercritical CO2 extraction oleanolic acid in the wrinkled papaya acid conditions. Methods: The content of oleanolic acid was determined by HPLC. The average yield and content of oleanolic acid were taken as indexes. The extraction temperature, extraction pressure, separation temperature, separation pressure and carrier type were investigated by single factor and orthogonal test Influencing factors, and comparative analysis of the preferred technology and literature technology. Results: The optimal extraction conditions of oleanolic acid by supercritical CO2 extraction were as follows: extraction temperature 45 ℃, extraction pressure 40 MPa, separation temperature 30 ℃, separation pressure 8 MPa, carrier 95% ethanol; supercritical CO2 extraction was superior to other methods . Conclusion: The optimal process is stable and feasible, which can be used for the popularization and application of oleanolic acid.