随着市场上半夏混淆品的盛行及半夏本身形态学的某些改变,使传统的形态学鉴别方法难度增加,必须结合其他方法才能准确鉴定。随着生物技术的发展,其在中药鉴定中的应用日渐突显,本文就目前常用于中药鉴别的生物技术方法进行了总结,主要可概括为色谱、分子生物学和电泳三大类,以期广泛应用于半夏真伪鉴别。 With the prevalence of Pinellia confusion in the market and certain changes in the morphology of Pinellia sinensis, the traditional method of identification of morphology has become more difficult and must be combined with other methods to accurately identify. With the development of biotechnology, its application in traditional Chinese medicine identification has become increasingly prominent. This article summarizes the biotechnology methods commonly used in the identification of traditional Chinese medicines, which can be summarized as three major categories of chromatography, molecular biology and electrophoresis, with a view to wide application. In the authentic identification of Pinellia.