论文部分内容阅读
多聚腺苷酸化(Polyadenylation)是真核生物中一种重要的pre-mRNA加工机制,是mRNA成熟的一个必经过程。为了解甘蓝BoKIN1基因的多聚腺苷酸化加工情况,本研究从甘蓝全基因组数据库中下载得到BoKIN1基因序列,并利用生物信息学手段分析了BoKIN1的基因结构和基因表达。研究结果显示:BoKIN1基因在花器官中表达最高;然后基于EST文库发现了3个ploy(A)位点,并计算了这些ploy(A)位点在premRNA加工过程中被选择的大致频率情况,结果显示BoKIN1基因在pre-mRNA加工过程中至少有6个ploy(A)位点可供选择加尾,其中AP4位点被选择加尾的频率最高,其次是AP5位点。因此选择AP4位点加尾后形成的mRNA是转录后加工的主要产物。通过本研究我们确定了甘蓝类BoKIN1基因6个选择性加尾位点及其3’UTR的主要产物和次要产物,可对于未来进一步利用这些3’UTR奠定基础。
Polyadenylation, an important pre-mRNA processing mechanism in eukaryotes, is a necessary process of mRNA maturation. In order to understand the polyadenylation of BoKIN1 gene in Brassica, BoKIN1 gene sequence was downloaded from the whole genome database of Brassica. BoKIN1 gene structure and gene expression were analyzed by bioinformatics analysis. The results showed that BoKIN1 gene was the highest expressed in floral organs. Then three ploy (A) sites were found based on EST library and the approximate frequency of these ploy (A) sites was selected during processing of premRNA. The results showed that there are at least 6 ploy (A) sites in BoKIN1 gene during the process of pre-mRNA processing. Among them, AP4 site was the most frequently selected, followed by AP5 site. Therefore, the selection of AP4 sites after the formation of mRNA is post-transcriptional processing of the main product. Through the present study, we identified six major selective and selective 3’UTR loci of BoKIN1 and their minor products, which laid the foundation for further utilization of these 3’UTR in the future.