论文部分内容阅读
目的研究1 800 MHz电磁辐射对细胞的氧化损伤作用。方法使用1 800 MHz,比吸收率为2、3、4 W/kg电磁辐射辐照小鼠皮肤细胞(JB6),细胞分为辐照组与对照组。辐照结束后,采用流式细胞术,DCFH-DA荧光探针检测细胞内活性氧(ROS)水平,再加入ROS中和剂富氢水,以观察其对ROS的中和作用。通过硫代巴比妥酸比色法检测丙二醛含量,来评价电磁辐射后细胞氧化损伤程度。结果比吸收率为2、3 W/kg时,暴露2h内细胞内ROS水平变化差异无统计学意义;4 W/kg暴露20 min、80 min、120min后,暴露组细胞与假暴露组比较ROS水平均显著升高(P<0.05);加入富氢水后,暴露组与普通培养基暴露组比较,降低了50.79%;4 W/kg暴露120min后丙二醛含量显著升高(P<0.05)。结论4 W/kg 1 800 MHz电磁辐射会引发细胞内ROS升高并造成细胞氧化损伤。
Objective To study the effect of 1 800 MHz electromagnetic radiation on oxidative damage of cells. Methods The mouse skin cells (JB6) were irradiated with electromagnetic radiation at 1 800 MHz and specific absorption rate of 2,3,4 W / kg. The cells were divided into irradiation group and control group. After the irradiation, the level of reactive oxygen species (ROS) in cells was detected by flow cytometry, DCFH-DA fluorescent probe, and ROS-neutralizing agent was added to ROS-neutralizing agent. By thiobarbituric acid colorimetric detection of malondialdehyde content to evaluate the extent of oxidative damage after electromagnetic radiation. Results When the specific absorption rate was 2 and 3 W / kg, there was no significant difference in intracellular ROS levels within 2 h of exposure. After exposed to 4 W / kg for 20 min, 80 min and 120 min, (P <0.05). After adding hydrogen-enriched water, the content of malondialdehyde in exposed group was 50.79% lower than that in normal medium exposure group; the content of malondialdehyde was significantly increased after exposed to 4 W / kg for 120min (P <0.05) ). Conclusion 4 W / kg 1 800 MHz electromagnetic radiation can induce intracellular ROS increase and oxidative damage of cells.