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[目的]研究高脂饲料(high fat diet,HFD)结合慢性应激(chronic stress,CS)致大鼠代谢综合征模型的特点。[方法]将32只雄性Wistar大鼠随机分为对照组、HFD喂养组、CS刺激组及HFD喂养结合CS刺激(HFD+CS)组,每组8只。持续处理12周后,检测胰岛素抵抗、血酯及血皮质醇水平,肝脏总胆固醇、三酰甘油总含量及水溶性成分含量,肝脏氧化应激、炎症程度及肝X受体α(liver X receptorα,LXRα)、过氧化物酶体增生物激活受体γ(peroxisomeproliferator-activated receptorγ,PPARγ)基因表达,内脏脂肪含量及其内分泌功能。[结果]慢性应激可恶化由高脂饲料引起的胰岛素抵抗、血脂紊乱、肝脏氧化应激、炎症及脂肪组织内分泌紊乱。该代谢综合征模型的明显特点是肝脏水溶性总胆固醇、三酰甘油含量明显升高,肝脏LXRα、PPARγmRNA表达进一步下调,血皮质醇浓度升高。[结论]高脂饲料结合慢性应激可建立具有典型代谢综合征特征的大鼠模型,慢性应激可恶化高脂饲料引起的代谢综合征病变。
[Objective] To investigate the characteristics of metabolic syndrome model induced by high fat diet (HFD) combined with chronic stress (CS). [Methods] 32 male Wistar rats were randomly divided into control group, HFD group, CS stimulation group and HFD + CS stimulation group (n = 8). After 12 weeks of continuous treatment, the levels of insulin resistance, blood ester and serum cortisol, liver total cholesterol, total triglycerides and water-soluble components, liver oxidative stress, inflammation and liver X receptor α , LXRα), peroxisome proliferator-activated receptorγ (PPARγ) gene expression, visceral fat content and its endocrine function. [Result] Chronic stress could aggravate insulin resistance, dyslipidemia, hepatic oxidative stress, inflammation and adipose tissue endocrine disorders caused by high fat diet. The metabolic syndrome model is characterized by liver water-soluble total cholesterol, triglyceride content was significantly increased liver LXRα, PPARγ mRNA expression was further decreased, blood cortisol levels increased. [Conclusion] High fat diet combined with chronic stress can establish a rat model with typical metabolic syndrome. Chronic stress can worsen the metabolic syndrome caused by high fat diet.