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通过OTC试验研究了生长60和120 d的转Bt水稻克螟稻及其对照亲本秀水11在2种CO2浓度(375、750μL·L-1)处理下的生理变化。结果表明:叶绿素a、叶绿素b、叶绿素a+b、可溶性蛋白含量以及谷氨酰胺合成酶活力等均随水稻生长而显著提高,游离氨基酸含量随水稻生长而显著下降,叶绿素a/b值在高CO2浓度下显著增加;CO2浓度升高会引起水稻组织含水量、叶绿素a和c含量显著降低,叶绿素b含量呈先增加后降低的趋势;同时,可溶性蛋白含量显著下降,游离氨基酸含量显著增加,谷氨酰胺合成酶活性初期变化不显著、后期显著降低;转Bt水稻及其对照亲本之间可溶性蛋白含量和谷氨酰胺合成酶活力差异显著,但叶绿素和游离氨基酸含量差异不显著;与亲本相比,不同CO2浓度处理下克螟稻的谷氨酰胺合成酶活力显著降低。
The physiological changes of Bombyx mori transgenic rice and its parental parent, Xiushui 11, treated with 2 CO2 concentrations (375 and 750 μL·L -1) at 60 and 120 d of growth were studied by OTC test. The results showed that the content of chlorophyll a, chlorophyll b, chlorophyll a + b, soluble protein and glutamine synthetase activity increased significantly with the growth of rice. The contents of free amino acids decreased significantly with the growth of rice and the chlorophyll a / b value was high The content of chlorophyll a and c decreased significantly while the content of chlorophyll b increased firstly and then decreased; at the same time, the content of soluble protein decreased significantly, the content of free amino acid increased significantly, The activity of glutamine synthetase did not change significantly at the initial stage and decreased significantly in the late stage. The contents of soluble protein and glutamine synthetase in transgenic Bt rice and its control were significantly different, but the contents of chlorophyll and free amino acids were not significantly different. Compared with the control, the activity of glutamine synthetase in Keerguita rice significantly decreased under different CO2 concentrations.