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【目的】紫外引起葡萄叶片芪类物质白藜芦醇积累是一种常见的辐射效应,研究积累与UV-C引起的氧化胁迫关系。【方法】采用UV-C对离体红地球葡萄叶片进行胁迫;应用荧光照相与HPLC分析芪类物质变化特征,利用RT-PCB分析其生物合成途径基因表达规律,DAB检测叶片活性氧水平,分光光度法测定丙二醛水平,依文思蓝检测叶片细胞死亡程度。【结果】以22.5(W.h)/cm~2UV-C辐射叶片后白藜芦醇的含量最大,达到158μg/g鲜重。同一叶片左右差异明显,辐射处理一侧芪类物质含量、活性氧水平和细胞死亡程度明显高于未处理一侧;经辐射后的叶片中的MDA含量高于对照组;加抗氧化剂CAT和NAC可明显降低UV-C对白藜芦醇诱导效果。【结论】UV-C辐射导致离体红地球葡萄叶片氧化胁迫受损,其变化在空间和时间上与芪类物质白藜芦醇积累密切相关。
【Objective】 The accumulation of resveratrol in grape leaves caused by UV was a common radiation effect, and the relationship between accumulation and UV-C-induced oxidative stress was studied. 【Method】 UV-C was used to inoculate grape leaves in vitro. The changes of stilbene content were analyzed by fluorescence and HPLC. The gene expression patterns of the biosynthetic pathways were analyzed by RT-PCR. The reactive oxygen species The level of malondialdehyde was measured by spectrophotometry and the degree of cell death was detected by Evans blue. 【Result】 The results showed that the highest content of resveratrol reached 158 μg / g fresh weight after radiated with 22.5 (W.h) / cm ~ 2 UV-C. The same leaf left and right significantly different, radiation treatment side of the content of stilbene, reactive oxygen species and cell death was significantly higher than the untreated side; irradiated leaves after the MDA content higher than the control group; plus antioxidant CAT and NAC Can significantly reduce the UV-C on resveratrol-induced effect. 【Conclusion】 UV-C radiation can lead to impaired oxidative stress in grape leaves of isolated red globe. The change of the oxidative stress is closely related to resveratrol accumulation in space and time.