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建立了榉树SRAP分子标记技术体系,并在此基础上结合毛细管电泳技术(CE,capillary electrophoresis)对来自贵州、江西、浙江和日本京都的70个榉树优良单株进行指纹图谱构建和遗传聚类分析。筛选出13对SRAP引物,共扩增出2276个位点,均为多态性条带,多态性百分率达到100%,筛选出的任意一条引物在采用毛细管电泳技术的情况下均可将所有样本区分开。70个优良单株间的遗传距离变化范围为0.0078~0.3772,平均值为0.0629;遗传相似系数在0.9218~0.9565之间,平均值为0.9339,说明种源间遗传距离近,遗传相似性较高。SRAP遗传聚类分析结果显示,榉树的分类依次与其所生长的时间、地理位置以及秋季叶色和叶面光滑程度等性状相关。本研究可以为榉树优良单株无性系的分子鉴定、遗传资源管理及品种保护提供参考依据。
The technical system of SRAP molecular marker for beech was established. On the basis of this, combined with capillary electrophoresis (CE), 70 elm trees from Guizhou, Jiangxi, Zhejiang and Kyoto, Japan, Class analysis Thirteen pairs of SRAP primers were screened and 2276 loci were amplified, all of which were polymorphic bands with the percentage of polymorphism reaching 100%. All of the selected primers could use the capillary electrophoresis technique The samples are separated. The genetic distance of 70 elite individuals varied from 0.0078 to 0.3772 with an average of 0.0629. The genetic similarity coefficient ranged from 0.9218 to 0.9565 with an average of 0.9339, indicating that the genetic distance between provenances was close and the genetic similarity was high. SRAP genetic clustering analysis showed that the classification of the beech tree in turn related to its growth time, geographical location and autumn leaf color and leaf smoothness and other traits. This study can provide a reference for the molecular identification, genetic resources management and variety protection of elite single plant clones of Beech.