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目的:观察地塞米松联合依达拉奉对百草枯中毒诱导的大鼠急性肺损伤(ALI)模型的治疗效果。方法:取健康成年雄性SD大鼠120只,随机分为生理盐水组(NS组)、百草枯组(PQ组)、百草枯+地塞米松组(实验1组),百草枯+地塞米松+依达拉奉组(实验2组),每组各30只。百草枯ALI模型,由百草枯溶液20mg/kg单次尾静脉注射制备;NS组则以等体积的生理盐水尾静脉注射所制。实验组是在百草枯模型成功制备1h后,以相应的药物处理得到,实验1组:在百草枯模型上予地塞米松1mg/kg腹腔注射所制;实验2组:在百草枯模型上给予地塞米松1mg/kg+依达拉奉3mg/kg腹腔注射所致。给药5h后处死大鼠,比较各组大鼠肺泡灌洗液中炎性细胞的数目、肺组织湿/干(W/D)、RT-PCR法检测肺组织中TNF-αmRNA及ELISA法检测血清中IL-1β、IL-8的水平。结果:PQ组肺泡灌洗液中炎性细胞数目、肺组织W/D、肺组织中TNF-αmRNA及血清中IL-1β、IL-8的水平均高于NS组(P<0.01);实验1、2组中的各项指标均低于PQ组(P<0.05、P<0.01);相较于实验1组,实验2组中肺泡灌洗液中炎性细胞数目、肺组织W/D均明显下降(P<0.01),肺组织中TNF-αmRNA及血清中IL-1β、IL-8的水平下降(P<0.05)。结论:地塞米松可缓解百草枯中毒所致的ALI,而联合依达拉奉,可增强这种保护作用。
Objective: To observe the therapeutic effect of dexamethasone and edaravone on acute lung injury (ALI) induced by paraquat poisoning in rats. Methods: One hundred and twenty healthy adult male Sprague-Dawley rats were randomly divided into normal saline group (NS group), paraquat group (PQ group), paraquat + dexamethasone group (experimental group 1), paraquat + dexamethasone + Edaravone group (experimental group 2), 30 in each group. Paraquat ALI model prepared by a single tail vein injection of paraquat solution 20mg / kg; NS group was injected with an equal volume of saline tail vein injection. The experimental group was prepared after the paraquat model was successfully prepared for 1h, and the corresponding drug treatment was used. In the experimental group 1, dexamethasone 1mg / kg was injected intraperitoneally on the paraquat model. In the experimental group 2, the paraquat model was given Dexamethasone 1mg / kg + edaravone 3mg / kg due to intraperitoneal injection. The rats were sacrificed 5h after the administration, the number of inflammatory cells in the bronchoalveolar lavage fluid, the wet / dry (W / D) of the lung tissue, the TNF-αmRNA in the lung tissue by RT-PCR and ELISA Serum IL-1β, IL-8 levels. Results: The number of inflammatory cells, W / D in lung tissue, TNF-αmRNA in lung tissue and levels of IL-1β and IL-8 in serum in BALF in PQ group were higher than those in NS group (P <0.01) The indexes in group 1 and 2 were lower than those in PQ group (P <0.05, P <0.01). Compared with experimental group 1, the number of inflammatory cells in bronchoalveolar lavage fluid, the ratio of W / D (P <0.01). The levels of TNF-αmRNA and IL-1β, IL-8 in serum decreased significantly (P <0.05). Conclusion: Dexamethasone can alleviate paraquat poisoning caused by ALI, and combined edaravone can enhance this protective effect.