Diatom oxygen isotopes have been widely applied in quantitative reconstruction of the paleoclimate and paleoenvironment,but have rarely been reported in China.In the present study,Lake Sihailongwan in Northeast China was selected for detailed investigation of oxygen isotopic fractionation between diatom frustules and lake water induced by growth temperature.This study involved a 2-year period of field monitoring of the lake water temperature at multiple depths and biweekly collections of traps for both sediment and water at shallow and bottom depths(7 and 49 m below the lake surface,respectively),diatom separation and purification of the trap sediments,and oxygen isotope measurement for diatom silica and lake water samples.The conditioned experiment conducted herein demonstrated that the laboratory device,methods and techniques used in this study were capable of generating reliable data for the typical oxygen isotope composition of diatom frustules.The data obtained revealed a prominent linear relationship between the oxygen isotope composition of the modern diatom and lake temperature during growth.The fractionation coefficient was about-0.185‰/°C-0.238‰/°C in the temperature range of 3.6-24°C,which is consistent with the observations from various aquatic environments and laboratory culture with different diatom taxa.These findings provide strong support for the dominant control of the growth temperature on the oxygen isotope fractionation between the diatom frustules and ambient water.A notable difference in the fractionation coefficient was observed between the surface and bottom diatom oxygen isotopes,suggesting that various depositional processes and taphonomic effects influenced the surface and bottom trap samples.Another factor leading to this difference may be that enrichment by evaporation and dilution by rainfall have a stronger influence on the surface.Overall,the results presented here demonstrate significant progress in evaluation of diatom oxygen isotopes in China and draw attention to the differences between surface and bottom diatom oxygen isotope compositions. Diatom oxygen isotopes have been widely applied in quantitative reconstruction of the paleoclimate and paleoenvironment, but have rarely been reported in China. The present study, Lake Sihailongwan in Northeast China was selected for detailed investigation of oxygen isotopic fractionation between diatom frustules and lake water induced by growth temperature. This study involved a 2-year period of field monitoring of the lake water temperature at multiple depths and biweekly collections of traps for both sediment and water at shallow and bottom depths (7 and 49 m below the lake surface, respectively) , diatom separation and purification of the trap sediments, and oxygen isotope measurement for diatom silica and lake water samples. conditioned that the data is for laboratory instruments, methods and techniques used in this study were capable of generating reliable data for the typical oxygen isotope composition of diatom frustules. the data obtained revealed a prominen t linear relationship between the oxygen isotope composition of the modern diatom and lake temperature during growth. The fractionation coefficient was about-0.185 ‰ / ° C - 0.238 ‰ / ° C in the temperature range of 3.6-24 ° C, which is consistent with the observations from various aquatic environments and laboratory culture with different diatom taxa. These findings provide strong support for the dominant control of the growth temperature on the oxygen isotope fractionation between the diatom frustules and ambient water. A notable difference in the fractionation coefficient was observed between the surface and bottom diatom oxygen isotopes, suggesting that various depositional processes and taphonomic effects influenced the surface and bottom trap samples. Another factor leading to this difference may be that enrichment by evaporation and dilution by rainfall have a stronger influence on the surface. Overall, the results presents here demonstrates significant progress in evaluation of diatom oxyge n isotopes in China and draw attention to the differences between surface and bottom diatom oxygen isotope compositions.