“炼苗”是组培育苗全过程中的一个重要环节，把握不好会直接影响组培苗的成活率。所以在“炼苗”时，应把握好以下三关。一、种苗关优质健壮的种苗是保证炼苗成活率的前提和基础。如果瓶苗在培养过程中因光照不足、温度不适、细胞分裂素含量过高、未经空白壮苗、生根不理想或 “Hardening” is an important part in the whole process of tissue culture seedlings. Poorly grasping will directly affect the survival rate of tissue culture seedlings. So in the “Lian Miao”, we should grasp the following three levels. First, seedling quality and robust seedling is to ensure the survival rate of refining the premise and basis. If the bottle seedlings in the training process due to inadequate lighting, temperature, cytokinin content is too high, without blank seedlings, not ideal or rooting