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目的:分析三七总皂苷对急性坏死性胰腺炎(ANP)大鼠NF-kB活性及肺胰腺损伤的影响。方法:选取SD大鼠36只,随机分成假手术组、对照组(急性坏死性胰腺炎组)及观察组(三七总皂苷处理组)各12例。假手术组和对照组在造模前1小时给予0.9%生理盐水腹腔注射,剂量0.1ml/100g,观察组造模前1小时给予三七总皂苷(浓度50mg/ml),剂量0.1ml/100g。对照组和观察组大鼠胆胰管逆行注射5%牛黄胆酸钠造模,假手术组未注牛黄胆酸钠,仅翻动十二指肠和胰腺关腹。每组均造模后6小时时大鼠处死,取肺组织及胰腺组织标本,测定NF-kB的活性,观察其病理特征。结果:对照组、观察组肺及胰腺组织中阳性细胞明显高于假手术组,P<0.05;观察组与对照组比较,阳性细胞明显减少,P<0.05;对照组、观察组肺及胰腺组织阳性单位明显高于假手术组,P<0.05;观察组阳性单位明显低于对照组,P<0.05;观察组肺及胰腺组织病理在充血、水肿、炎性细胞的浸润等方面均轻于对照组。结论:在ANP大鼠的肺及胰腺组织中,NF-kB活性明显被激活,三七总皂苷用于急性坏死性胰腺炎大鼠中,能抑制肺及胰腺组织NF-kB的活性,并使其病理损伤减轻,有望用于ANP的治疗。
Objective: To analyze the effect of Panax notoginseng saponins on the activity of NF-κB and the injury of lung and pancreas in rats with acute necrotizing pancreatitis (ANP). Methods: Thirty-six SD rats were randomly divided into sham operation group, control group (Acute necrotizing pancreatitis group) and observation group (Panax notoginseng total saponin treatment group), 12 cases each. Sham operation group and control group were given intraperitoneal 0.9% saline intraperitoneal injection 0.9% saline 1 hour before model making. The observation group was given Panax notoginsenoside (concentration 50mg / ml) one hour before model making, the dosage was 0.1ml / 100g . Rats in control group and observation group were retrospectively injected with 5% sodium taurocholate into the biliary and pancreatic ducts. The rats in sham operation group were given sodium taurocholate, but only the duodenum and the pancreas were closed. Rats in each group were sacrificed at 6 hours after the model was established. Specimens of lung and pancreas were taken and the activity of NF-kB was measured. The pathological features were observed. Results: The positive cells in the lung and pancreas of the control group and the observation group were significantly higher than those of the sham operation group (P <0.05); the number of positive cells in the observation group and the control group was significantly decreased (P <0.05); in the control and observation groups, The positive unit was significantly higher than the sham operation group, P <0.05; the positive unit in the observation group was significantly lower than the control group, P <0.05; the pathological changes of the lung and pancreas in the observation group were lighter than the control in the congestion, edema and inflammatory cell infiltration group. Conclusion: NF-κB activity is obviously activated in the lung and pancreas of ANP rats. Panax notoginseng saponins can inhibit the activity of NF-κB in lung and pancreas tissues in rats with acute necrotizing pancreatitis Its pathological damage alleviated, is expected to be used for the treatment of ANP.