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本文采用骨形态计量学、扫描电镜—能谱分析(SEM-EDXA)等方法综合评价成年去势大鼠骨丢失情况。结果显示:OVX组与sham组比较,胫骨小梁体积明显降低.小梁显著变窄,类骨质体积增加,骨形成和骨吸收均增强,但吸收大于形成;胫骨SEM-EDXA检测Ca、P元素x线光子数分别降低50%;肱骨湿重、干重、灰重与体重之比明显下降,骨钙含量显著减少;血清磷明显升高、AKP升高16.2%,血清雌二醇含量显著降低等。以上多种方法检测结果均证实,4月龄SD大鼠切除卵巢后10周,由于雌激素缺乏,造成了典型的绝经后骨质硫松动物模型,可广泛用作抗骨质疏松药物的体内筛选。
In this study, the bone loss of adult ovariectomized rats was evaluated synthetically using methods such as bone morphometry and scanning electron microscopy-energy dispersive spectroscopy (SEM-EDXA). The results showed that compared with the sham group, the trabecular bone volume was significantly reduced in the OVX group and the trabecular bone was significantly narrower, the bone-like volume was increased, the bone formation and bone resorption were both enhanced, but the absorption was greater than the formation; the skeletal bone SEM-EDXA detection Ca, P The element x-ray photon number decreased by 50%; the ratio of wet weight, dry weight, ash weight to body weight of iliac bone significantly decreased, bone calcium content decreased significantly; serum phosphorus increased significantly, AKP increased by 16.2%, serum estradiol content was significant Lower etc. The test results of the above methods all confirmed that a typical postmenopausal osteoporosis bone model was created in the 4 weeks after ovariectomy in 4 month old SD rats due to lack of estrogen, which can be widely used as an anti-osteoporosis drug in vivo. filter.