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以对青枯病抗性不同的两个玉米自交系为材料,研究青枯病对玉米叶片过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、叶绿素含量、可溶性蛋白含量以及电导率的影响。结果表明,接种青枯病菌后,感病自交系黄早四从14 d开始出现感病症状,抗病自交系K12HF526在30 d后才出现轻微感病症状。两个自交系对照组叶片的POD活性、SOD活性和MDA含量表现趋势一致,抗病自交系高于感病自交系。不同自交系对照组的叶片叶绿素含量一致,处理组中感病自交系明显低于抗病自交系,可能是感染青枯病导致叶绿素含量降低。抗病自交系叶片可溶性蛋白含量在接种前后差异不显著,感病自交系接种21 d后显著降低。通过电导率计算伤害率的结果表明,抗性自交系的伤害率远远低于感病自交系。
Two maize inbred lines with different resistance to bacterial wilt were used as materials to study the effects of bacterial wilt on the activities of peroxidase (POD), superoxide dismutase (SOD), malondialdehyde (MDA) Content, chlorophyll content, soluble protein content and conductivity. The results showed that the susceptible inbred line Huangzao 4 began to show symptoms after 14 days inoculation with the inoculated wilt bacterium, and the susceptible inbred line K12HF526 showed slight symptoms after 30 days. The trend of POD activity, SOD activity and MDA content in leaves of two inbred lines were the same, and the resistant inbred lines were higher than susceptible inbred lines. The leaf chlorophyll content of different inbred lines was the same, and the susceptible inbred lines in the treatment group were significantly lower than the resistant inbred lines, which may be caused by the infection of bacterial wilt and the decrease of chlorophyll content. The content of soluble protein in leaves of resistant inbred lines was not significantly different before and after inoculation, and the susceptible inbred lines significantly decreased after 21 d of inoculation. The results of damage rate calculated by conductivity showed that the injury rate of resistant inbred lines was much lower than that of susceptible inbred lines.