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采用外源DNA 导入技术,研究了DNA 种苗浸渍对小麦种子发茅的影响,颖花滴注对结实率的影响以及外源DNA 导入受体诱导变异的效果。结果表明,外源DNA 浸种对发芽生根有不良的影响。浸种时DNA 稀释液的浓度不应大于0.1×ssc。DNA 液颖花滴注可获得较高的结实率。外源DNA 导入受体后产生了具有目的性状的变异(如粒色、芒长、不育性等)和非供体性状的变异(如株带蜡质、抽穗迟等)。而且大麦DNA 导入小麦后,成功地获得了高抗白粉病变异株。
The effects of DNA seedling immersion on the germination of wheat seeds, the effect of spikelet on the seed setting rate, and the induced mutation of exogenous DNA into the recipient were studied by using exogenous DNA introduction technology. The results showed that the exogenous DNA soaking germination on the root has a negative impact. Soaking DNA dilution concentration should not exceed 0.1 × ssc. DNA liquid spike infusion can get higher seed setting rate. Introduction of exogenous DNA into the recipient resulted in variation (such as grain color, mantle length, sterility, etc.) and non-donor variation (such as banding waxy, late heading) with the trait of interest. Moreover, barley DNA was successfully transformed into powdery mildew resistant wheat lines after it was introduced into wheat.