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作者用原代人胚肾细胞从46个患者分离出B组腺病毒(Ad),经Sma Ⅰ酶解病毒DNA分析,证明这些病毒均为Ad35。这些病人中36人为AIDS或AIDS相关综合征(ARC)患者,7人由于其他疾病引起免疫抑制,只有3人是无免疫抑制证据的“正常人”。用中和试验方法测定患者血清Ad35和Ad2特异性抗体,后者作为以前受过Ad感染的标志。除在1名接受肾移植患者和1名免疫功能正常患者检测到特异性Ad35抗体外,在2名AIDS和3名ARC患者均未检测到Ad35特
The authors isolated B group of adenovirus (Ad) from 46 patients with primary human embryo kidney cells and analyzed by SmaI enzymatic digestion of the viral DNA to confirm that all of these viruses are Ad35. Of these patients, 36 were AIDS or AIDS-related complex (ARC) patients, 7 were immunosuppressed due to other diseases and only 3 were “normal people” with no evidence of immunosuppression. Patient serum Ad35 and Ad2 specific antibodies were assayed by the neutralization assay, which was used as a marker of previous infection with Ad. Ad35 was not detected in 2 AIDS and 3 ARC patients except one specific Ad35 antibody in one kidney transplant patient and one immunocompetent patient