Dear Editor,rnThe scarce number of secondary spermatocytes in human seminiferous tubules and the complexity of analyzing chromosomes at metaphase II are the main causes of the limited knowledge we have about chromosome characteristics at this stage. In this study, we have performed a comprehensive analysis of meiotic abnormalities in metaphase II spermatocytes combining Leishman\'s staining protocols and multiplex fluorescent in situ hybridization procedures. Results indicated that infertile individuals showed different susceptibilities to meiotic abnormalities in secondary spermatocytes without a preferentially effect on any particular chromosome.