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目的探讨邻苯二甲酸二(2-乙基己基)酯(DEHP)和氯氰菊酯(CYP)单独及联合染毒对青春前期雄性大鼠生殖发育的不良影响。方法选择SPF级3周龄雄性SD大鼠24只,随机分为4组,分别为对照组(喂饲玉米油)、DEHP染毒组(500mg/kg)、CYP染毒组(80mg/kg);DEHP、CYP联合染毒组(DEHP 500mg/kg+CYP 80mg/kg),每组6只。采用经口灌胃方式染毒,每天1次,连续染毒30天。于末次染毒24小时后处死动物,测定大鼠体重、睾丸湿重,并计算睾丸系数;测定血清睾酮水平;制备睾丸病理组织切片,光镜观察其组织学改变,电镜观察生殖细胞超微结构;测定睾丸标志酶乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、碱性磷酸酶(ALP)和琥珀酸脱氢酶(SDH)的活性。结果与对照组相比,DEHP、CYP单独及联合染毒组睾丸下降时间和包皮分离时间明显延迟,肛门生殖器间距明显缩短(P<0.05或0.01);DEHP单独染毒组睾丸重量及睾丸系数明显降低,血清睾酮水平显著下降(P<0.05),睾丸匀浆中ALP、ACP和LDH活性明显增高,SDH活性显著下降(P<0.01);DEHP、CYP单独及联合染毒组睾丸病理组织学、生殖细胞超微结构均可见明显异常。结论 DEHP、CYP单独及联合染毒对青春前期雄性大鼠均具有明显的生殖毒性作用,可引起生精细胞、支持细胞和间质细胞的变性、坏死及功能障碍,从而导致雄性生殖系统发育和功能的显著异常。其中,DEHP单独染毒呈现主效应,DEHP、CYP联合染毒对大鼠的生殖毒性未呈现明显交互影响。
Objective To investigate the adverse effects of DEHP and cypermethrin (CYP) alone and in combination on the reproductive development of preadolescent male rats. Methods Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups: control group (fed with corn oil), DEHP group (500mg / kg), CYP group (80mg / kg) ; DEHP and CYP combined treatment group (DEHP 500mg / kg + CYP 80mg / kg), 6 in each group. Using oral gavage poisoning, 1 day, 30 consecutive days of exposure. The animals were sacrificed 24 hours after the last exposure. The body weight and wet weight of testis were determined. The testicular coefficient was calculated. The level of serum testosterone was measured. The histopathological sections of testis were prepared. The histological changes were observed under light microscope. The ultrastructure of germ cells The activities of testicular markers LDH, ACP, ALP and SDH were determined. Results Compared with the control group, the descending time and prepuce separation time of DEHP and CYP groups were significantly delayed and the anogenital spacing was significantly shortened (P <0.05 or 0.01). The testis weight and testicular coefficient of DEHP group were significantly higher than those of control group (P <0.05). The activities of ALP, ACP and LDH in testis homogenate were significantly increased and the activities of SDH were significantly decreased (P <0.01). The histopathological changes of testis such as DEHP and CYP, Germ cell ultrastructure showed obvious abnormalities. Conclusion DEHP and CYP alone and in combination have significant reproductive toxic effects on pre-adolescent male rats, which may cause degeneration, necrosis and dysfunction of spermatogenic cells, supporting cells and interstitial cells, leading to male reproductive system development and Significant abnormalities in function. Among them, DEHP alone showed the main effect of exposure, DEHP, CYP combined exposure on reproductive toxicity of rats showed no significant interaction.