cDNA library of wheat near isogenic line TcLr19 was constructed with suppression subtractive hybridization (SSH) 16 h after inoculation with race 366 of Puccinia recondita f. sp. tritici. This SSH library included 1337 positive clones and the insert sizes ranged from 200 bp to 600 bp, 237 clones were selected according to the result of reverse northe blotting, and then 35 ESTs were sequenced. EST similarity analysis was finished by comparing sequences with BLAST software in the non redundant database of GenBank. The results showed that they were related to many biological processes including signal transduction, transcription regulation and hypersensitive response.