论文部分内容阅读
为了提高高速逆流色谱仪的固相保留率,扩大逆流色谱仪的应用范围,本文通过结合物理、数学知识,分析固-液摩擦力、液-液摩擦力之间的相互影响,并通过数学模型验证了平面螺旋柱的切向离心力作用。此三种力共同决定了流动相的在柱内流速的改变,从而影响到柱内流动相的横截面积,继而影响到固定相的横截面积,最终决定了固相保留率的大小;并通过实验结果加以验证,得出F-H-U与R-H-U两种模式的固相保留效果最好。
In order to improve the solid phase retention rate of high speed countercurrent chromatography and expand the application range of countercurrent chromatography, this paper analyzes the interaction between solid-liquid friction and liquid-liquid friction through the combination of physical and mathematical knowledge, and through the mathematical model The tangential centrifugal force of the planar helical column was verified. Together, these three forces determine the change in the flow velocity of the mobile phase within the column, which in turn affects the cross-sectional area of the mobile phase in the column, which in turn affects the cross-sectional area of the stationary phase, which ultimately determines the size of the solid phase retention rate; Through the experimental results to be verified, FHU and RHU obtained two solid phase retention mode best.