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在组建成功人白细胞介素4(hIL 4)表达质粒PBV 220/hIL 4基础上,应用多聚酶链式反应(PCR)技术、构建成hIL 4高效表达克隆,命名为PBV220/hIL4a。核酸序列分析证明该质粒不但去除了hIL 4终止密码TGA后200bp的3′末端侧翼区核苷酸,还以定位突变技术改原终止密码TGA为原核系统强终止密
Based on the construction of hIL 4 expression plasmid PBV 220 / hIL 4, the hIL 4 highly expressed clone named PBV220 / hIL4a was constructed by polymerase chain reaction (PCR). Nucleic acid sequence analysis proved that this plasmid not only removed 200 bp of the flanking region nucleotide of 200 bp after the TIL of hIL 4 stop codon, but also terminated the codon by the site-directed mutagenesis technique.