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目的:观察赤雹根总皂苷的镇痛作用,探讨赤雹根镇痛作用的有效部位。方法:采用小鼠48℃热板法、小鼠48℃热水浸尾法、灌胃酒石酸锑钾致小鼠扭体法,评价赤雹根总皂苷的镇痛作用。结果:48℃热板试验中,赤雹根总皂苷各剂量组(240mg/kg、120mg/kg、60mg/kg)小鼠痛反应潜伏期(35.73±3.84)s、(28.20±3.74)s、(27.00±3.38)s均显著高于NS对照组(24.88±5.02)s,且差异有统计学意义(P<0.05～0.01)。小鼠48℃甩尾实验中,赤雹根总皂苷各剂量组药后40min、60min缩尾潜伏期(11.89±1.36)s、(10.83±1.09)s、(10.30±2.20)s、(8.98±1.65)s、(9.08±1.22)s、(8.01±2.04)s均显著高于NS组(7.27±1.32)s,且差异有统计学意义(P<0.05)。酒石酸锑钾致小鼠扭体反应实验中,赤雹根总皂苷各剂量组15min内小鼠扭体反应次数(24.10±2.77)次、(28.40±3.75)次、(35.60±4.99)次均显著低于NS组(40.40±5.04)次,且差异有统计学意义(P<0.05～0.01)。结论:赤雹根总皂苷具有显著的镇痛作用。
OBJECTIVE: To observe the analgesic effect of total saponins from hail-hail and explore the effective site of the analgesic effect of hail-hail. Methods: The analgesic effect of total saponins of Hail Haematoxylin was evaluated by mouse hot plate method at 48 ℃, hot water immersion at 48 ℃ in mice and writhing mice induced by antimony potassium tartrate. Results: In the hot plate test at 48 ℃, the pain reaction latency (35.73 ± 3.84) s, (28.20 ± 3.74) s, ( 27.00 ± 3.38) s were significantly higher than NS control group (24.88 ± 5.02) s, and the difference was statistically significant (P <0.05 ~ 0.01). In mice tail-flick experiment at 48 ℃, the tail loss latency (11.89 ± 1.36) s, (10.83 ± 1.09) s, (10.30 ± 2.20) s, (8.98 ± 1.65), ) s, (9.08 ± 1.22) s and (8.01 ± 2.04) s were significantly higher than those in NS group (7.27 ± 1.32) s, respectively, and the difference was statistically significant (P <0.05). In writhing reaction of mice induced by antimony potassium tartrate, the number of writhing reaction (24.10 ± 2.77) times (28.40 ± 3.75) times and (35.60 ± 4.99) times in each dose of red haystone total saponins were significantly Lower than NS group (40.40 ± 5.04) times, and the difference was statistically significant (P <0.05 ~ 0.01). Conclusion: The total saponins of red hail gum have a significant analgesic effect.