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采用改进的Hummer方法合成氧化石墨烯,通过控制超声时间获得不同横向尺寸的纳米片,将其与大肠杆菌作用,发现氧化石墨烯的抗菌活性与其横向尺寸呈正相关,当氧化石墨烯平均横向尺寸为2.5μm时,大肠杆菌的失活率达到96.4%.以革兰氏阴性菌大肠杆菌、绿脓杆菌和革兰氏阳性菌金黄色葡萄球菌作为研究对象,考察氧化石墨烯的抗菌性能.结果表明,低浓度(≤100μg·mL~(-1))时氧化石墨烯对革兰氏阴性菌的抑制作用强于对革兰氏阳性菌的抑制作用,当浓度达到200μg·mL~(-1)时,3种细菌失活率均能达到90%以上.最后通过原子力显微镜(AFM)观察大肠杆菌在氧化石墨烯作用下随时间的变化,发现大肠杆菌被氧化石墨烯纳米片包裹,其抗菌作用推测是通过氧化石墨烯片的氧化应激和阻隔营养物质的输送实现.
The modified Hummer method was used to synthesize graphene oxide. The nanosheets with different lateral sizes were obtained by controlling the ultrasonic time and interacting with Escherichia coli. The results showed that the antibacterial activity of graphene oxide was positively correlated with its lateral size. When the average lateral size of graphene oxide was The inactivation rate of Escherichia coli reached 96.4% at 2.5μm.The Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus were investigated as antibacterial properties of Graphene oxide Gram-negative bacteria were more sensitive to Gram-negative bacteria than Gram-positive bacteria at low concentration (≤100μg · mL -1). When the concentration reached 200μg · mL -1, , The inactivation rates of all three kinds of bacteria can reach more than 90% .Finally, the changes of Escherichia coli under the action of graphene oxide (OMG) by atomic force microscope (AFM) were observed. The results showed that E. coli was coated with graphene oxide nanosheets and its antibacterial activity It is presumed to be achieved by oxidative stress of graphene oxide sheets and blocking the delivery of nutrients.