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目的:研究柯萨奇B3病毒(CVB3)/MKP株5’非编码区基因序列及变异性,探讨其致病性的分子基础。方法:用Hela细胞培养柯萨奇B3病毒,RT-PCR法扩增5’非编码区,测序后进行同源性、变异性及遗传进化分析。结果:CVB3/MKP株5’非编码区基因全长742个核苷酸,该株病毒与CVB3/20、CVB3/28、CVB3/0、CVB3/Nancy、CVB3/AS、CVB3/Woodruff(CVB3/W)及CVB3(CXA3CG)株5’非编码区同源性在91.9%～99.6%之间,而与CVB3/GA和CVB3/CO两种病毒株同源性分别为83.6%和83.3%。CVB3/MKP株的5’非编码区序列与既致胰腺炎又致心肌炎的病毒株共同聚簇,而与非致病毒株和仅致胰腺炎的病毒株距离较远。结论:CVB3/MKP株5’非编码区基因序列可能与病毒的毒力表型有关。
Objective: To study the sequence and variability of 5 ’non-coding region of coxsackievirus B3 (CVB3) / MKP strain and to explore the molecular basis of its pathogenicity. Methods: Coxsackie B3 virus was cultured in Hela cells. The 5 ’untranslated region was amplified by RT-PCR. The sequences were sequenced and analyzed for homology, variability and genetic evolution. Results: The 5 ’non-coding region of CVB3 / MKP strain was 742 nucleotides in length. The CVB3 / 20, CVB3 / 28, CVB3 / 0, CVB3 / Nancy, CVB3 / W and CVB3 (CXA3CG) strains shared 91.9% -99.6% identity with the 5 ’untranslated regions of CVB3 (CVA3CG), while the homologies with CVB3 / GA and CVB3 / CO strains were 83.6% and 83.3%, respectively. The 5 ’non-coding region of the CVB3 / MKP strain co-clustered with both the pancreatic and myocarditis-causing strains, but far away from the non-virulent and pancreatic-only strains. Conclusion: The 5 ’non-coding region of CVB3 / MKP strain may be related to virulence phenotype.