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目的 观察雌激素受体基因型与妇女骨密度的关系。方法 采用双能Ｘ线吸收骨密度仪测量 78例绝经后妇女、2 3例围绝经期妇女和 52例 2 5～ 3 5岁妇女的骨密度 ,用聚合酶链反应限制性片段长度多态 (ＰＣＲ ＲＦＬＰｓ)方法分析雌激素受体基因型。结果 在 78例绝经后妇女中 ,各种基因型的骨密度差异无显著性 (Ｐ >0 0 5)。在 52例年轻妇女中 ,仅在Ｗａｒｄ三角 ,ｐｐ型的骨密度(0 82 3± 0 0 95)ｇ/ｃｍ2 ,较ＰＰ型的 (0 665± 0 0 71 )ｇ/ｃｍ2 高 (Ｐ =0 0 3 7) ,其他部位各基因型间的骨密度差异无显著性 (Ｐ >0 0 5) ;联合分析ＰｖｕⅡ和ＸｂａⅠ位点 ,ｘｘｐｐ型的全身、腰椎、大转子、髋部和Ｗａｒｄ三角的骨密度比其他几种组合基因型的骨密度高 (Ｐ均 < 0 0 5) ;在绝经后妇女中 ,各种组合基因型的骨密度差异无显著性 (Ｐ均 >0 0 5)。结论 基因型ｘｘｐｐ型可能提示腰椎、大转子、髋部和Ｗａｒｄ三角的峰值骨密度较高 ,但雌激素受体基因型与绝经后妇女的骨密度无相关性。
Objective To observe the relationship between estrogen receptor genotypes and bone mineral density in women. Methods BMD of 78 cases of postmenopausal women, 23 cases of perimenopausal women and 52 cases of 25 ~ 35 years old women were measured by dual energy X-ray absorptiometry. The polymerase chain reaction restriction fragment length polymorphism PCR RFLPs) method to analyze estrogen receptor genotypes. Results In 78 postmenopausal women, there was no significant difference in BMD among various genotypes (P> 0.05). In 52 young women, the pp-type had a higher BMD (0 82 3 ± 0 0 95) g / cm2 than the PP-type (0 665 ± 0 071) g / cm2 There was no significant difference in BMD between genotypes in other sites (P> 0.05). The genotypes of PvuII and XbaI, xxpp type body, lumbar spine, greater trochanter, hip and Ward triangle Bone mineral density was higher than those of other genotypes (P <0.05). There was no significant difference in bone mineral density between the various genotypes in postmenopausal women (P> 0.05). Conclusions The genotype xxpp may indicate a higher peak BMD in the lumbar spine, greater trochanter, hip and Ward triangle, but there is no correlation between estrogen receptor genotypes and BMD in postmenopausal women.