论文部分内容阅读
目的 探讨葡萄籽原花青素对H2O2导致的人晶状体上皮细胞损伤的保护作用的机制.方法对人晶状体上皮细胞进行培养, 分别加入设计浓度的原花青素培养液, 经过相应的培养时间后, 再与含有不同浓度过氧化氢的培养液共同作用.流式细胞仪检测葡萄籽原花青素对细胞内活性氧的影响.用Western blot方法检测各组细胞中p38, p-p38, JNK, p-JNK蛋白的变化.结果 在体外HLE-B3细胞培养状态下, 葡萄籽原花青素能有效地降低细胞内活性氧水平.蛋白测定表明, p-p38, p-JNK蛋白表达下降.结论 葡萄籽原花青素能够有效抑制p-p38, p-JNK蛋白表达, 阻断了MAPK细胞信号传导通路, 减少氧化氢诱导的氧化损伤的发生, 从而对人晶状体上皮细胞具有保护作用.“,”Objective To investigate the protective effect of grape seed proanthocyanidin extract (GSPE) on H2O2-induced oxidative stress in human lens epithelial B-3 (HLEB-3) cells and the possible molecular mechanism involved.Methods After HLE-B3 cells grown to 75%~ 80% confluence, cells were pretreated with GSPE.After incubation for the indicated time, cells were treated with H2O2 in combination.The ROS generation was detected by flow cytometric analysis.Expression of mitogen activated protein kinase (MAPK) proteins was measured by Western blot.Results GSPE clearly reduced H2O2-induced cell reactive oxygen species (ROS) generation and protected HLEB-3 cells from H2O2 induced oxidative damage.H2O2-induced phosphorylation of the p38 and c-Jun N-terminal kinase (JNK) proteins of the MAPK family were decreased at various time points.Conclusion GSPE could be useful in attenuation of H2O2-induced oxidative stress and the activation of MAPK signaling in HLE-B3 cells, which suggests that GSPE has a potential protective effect against cataractogenesis.