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将丝素蛋白分别溶解在甲酸和六氟异丙醇(HFIP)内,通过静电纺丝方法成功纺制丝素(SF)纳米非织造网。其中以甲酸为溶剂所纺纳米纤维的平均直径是80nm,并且直径分布呈单峰状,而以HFIP为溶剂的纳米纤维直径较粗为380nm。将SF纳米非织造网浸在甲醇水溶液中进行后处理,并通过红外光谱和13CCP-MAS固体NMR谱研究非织造网的结构转变。以甲酸为溶剂制备的SF纳米纤维具有更多的β-折叠构象。甲醇水溶液是改变SF纳米纤维二级结构从无规卷曲到β-折叠结构转变的快速又有效的方法。目前的研究发现,通过使用不同溶剂可以控制丝素蛋白的尺寸和二级结构,从而将材料应用于生物医学方面,特别是组织工程方面。
Silk fibroin was dissolved in formic acid and hexafluoroisopropanol (HFIP), respectively, and spun silk (SF) nanofiber nonwoven was successfully spun by electrospinning. Among them, the average diameter of nanofibers spinning with formic acid as solvent was 80nm, and the diameter distribution was single peaked while that of nanofibers with HFIP as solvent was 380nm. The SF nano-nonwoven web was immersed in methanol aqueous solution for post-treatment, and the structural transformation of the nonwoven web was investigated by infrared spectroscopy and 13CCP-MAS solid-state NMR spectroscopy. SF nanofibers prepared with formic acid as solvent have more β-sheet conformations. Methanol aqueous solution is a fast and effective method to change the secondary structure of SF nanofibers from random coil to β-sheet structure. The current study found that by using different solvents can control the size and secondary structure of silk fibroin, which will be applied to biomedical materials, especially in tissue engineering.