本文介绍一种具有高度选择性的新方法,即应用包被有特异性抗原的磁性微球来快速筛选和克隆分泌特异性抗体的杂交瘤细胞.用人绒毛膜促性腺激素(hCG)作免疫原免疫6～12周龄雌性BALB/c小鼠,免疫脾细胞按常规方法与SP2/0骨髓瘤细胞融合.融合剂是聚乙二醇(PEG)与二甲亚砜(DMSO)的混合物.细胞在HAT选择培养基里培养7～10天后,先用ELISA法初步确 This article describes a highly selective new method for the rapid screening and cloning of hybridoma cells secreting specific antibodies using magnetic microspheres coated with specific antigens. Human chorionic gonadotropin (hCG) was used as immunogen Female BALB / c mice, 6-12 weeks old, were immunized and immunized splenocytes were fused to SP2 / 0 myeloma cells by conventional methods.The fusogenic agent was a mixture of polyethylene glycol (PEG) and dimethyl sulfoxide (DMSO) After culturing for 7-10 days in HAT selective medium, the method of ELISA was initially used