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许多研究表明:人早期胎盘是一个复杂的神经内分泌器官.我们已观察到人早期胎盘中不仅存在多种调节肽,而且存在5-羟色胺(5-HT),多巴胺(DA)和去甲肾上腺素(NE)等多种单胺类神经递质;它们在胚泡着床和早期妊娠的维持中起着重要的作用.但有关人早期胎盘绒毛中单胺类神经递质受体的研究,迄今未见报道.作者最近已成功地制备了DA和NE抗独特型抗体,并证实它们分别对DA和NE受体具有特异性免疫反应.本实验用以上两种抗独特型抗体的免疫组织化学法,探讨了DA和NE受体在人早期胎盘绒毛中的分布,为研究该两种神经递质在胎盘中的作用及其机理提供形态学依据.1 材料与方法1.1 试剂兔DA和NE抗独特型抗体(Anti-idiotypic antibody)均为中国科学院动物研究所本实验室自制;过氧化物酶-抗过氧化物酶复合物(PAP),由第四军医大学组织胚胎教研室提供.1.2 胎盘绒毛石蜡切片的制备由人工流产胚胎取得新鲜的胎盘绒毛(7～8周)6例(由北京市海淀医院计划生育门诊室提供),立即用生理盐水洗去血液,剪成小块,然后用Bouin液固定;石蜡包埋,制成5～7μm的连续切片.
Many studies have shown that human early placenta is a complex neuroendocrine organ.We have observed that there are not only multiple regulatory peptides present in the early placenta but also serotonin (5-HT), dopamine (DA) and norepinephrine (NE) and other monoamine neurotransmitters, which play an important role in blastocyst implantation and early pregnancy maintenance, but studies of monoamine neurotransmitter receptors in human early placental villi have so far Has not been reported.The authors have recently successfully prepared DA and NE anti-idiotypic antibodies and confirmed that they have specific immune response to DA and NE receptors.In this experiment, the two anti-idiotypic antibody immunohistochemistry , To explore the distribution of DA and NE receptors in early human placenta, and to provide a morphological basis for the study of the role and mechanism of the two neurotransmitters in the placenta.1 Materials and Methods 1.1 Reagents Rabbits DA and NE anti-unique Anti-idiotypic antibodies are all self-made by the Institute of Zoology, Chinese Academy of Sciences. Peroxidase-peroxidase complex (PAP) is provided by Department of Embryology, Fourth Military Medical University .1.2 Placenta paraffin Slice system Six cases of fresh placental villi (from 7 to 8 weeks) were obtained from aborted fetuses (provided by family planning clinic of Beijing Haidian Hospital), blood was immediately washed with saline, cut into small pieces, and then fixed with Bouin solution; paraffin Embedded, made of 5 ~ 7μm serial sections.