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目的观察化合物zome wermel 1-2(ZW1-2)对小鼠永久性局灶脑缺血后的神经功能,以及对脑源性营养因子和血管内皮生长因子的影响。方法制备小鼠永久性局灶脑缺血模型,并分别于脑缺血后2.5 h和7.5 h,灌胃给予不同剂量的化合物ZW1-2,脑缺血后24 h采用免疫组化法测定小鼠各个脑缺血易损区的脑源性神经营养因子和血管内皮生长因子表达情况。结果 ZW1-2能够显著降低小鼠局灶性脑缺血导致的行为功能评分,可以显著提高皮质、纹状体和海马脑区的脑源性神经营养因子表达,显著降低这些脑区中的血管内皮生长因子蛋白表达。结论 ZW1-2具有抗实验性脑缺血作用,其作用机制可能通过调控脑源性神经营养因子以及血管内皮生长因子而起到对脑缺血损伤的治疗作用。
Objective To observe the neurological function of compound zome wermel 1-2 (ZW1-2) after permanent focal cerebral ischemia in mice and its effect on brain-derived trophic factor and vascular endothelial growth factor. Methods The model of permanent focal cerebral ischemia in mice was established. The rats were administered with different doses of compound ZW1-2 intragastrically at 2.5 h and 7.5 h after cerebral ischemia, respectively. Immunohistochemistry Expression of brain - derived neurotrophic factor and vascular endothelial growth factor in various brain ischemic vulnerable areas in rats. Results ZW1-2 significantly decreased the scores of behavioral function induced by focal cerebral ischemia in mice and significantly increased the expression of BDNF in cortex, striatum and hippocampus and significantly decreased the blood vessels in these brain regions Endothelial growth factor protein expression. Conclusion ZW1-2 has anti-experimental cerebral ischemia, and its mechanism may play a therapeutic role in cerebral ischemic injury by regulating brain-derived neurotrophic factor and vascular endothelial growth factor.