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目的:观察凋亡相关因子半胱氨酸天冬氨酸蛋白酶8(caspase-8)和半胱氨酸天冬氨酸蛋白酶9(caspase-9)在肝细胞癌中的表达及临床病理意义。方法:选择我院自2012年7月~2013年4月的53例肝细胞肝癌患者作为观察组,选择同一时期在医院体检的50例正常肝组织患者作为对照组,采用原位分子杂交方法对两组患者的Caspase-8和Caspase-9mRNA进行检测。结果:观察组与对照组中caspase-8、caspase-9的阳性表达率分别为84.91%(45/53)、88.68%(47/53)和60.00%(30/50)、82.00%(41/50),观察组与对照组比较均有升高趋势,比值有显著性差异(P<0.05)。结论:肝癌细胞中caspase-8、caspase-9的较高表达显示在肝癌的发生发展过程中起到重要作用,在肝癌组织细胞增殖凋亡中具有一定作用,肝癌中caspase-8和caspase-9蛋白的表达均对于判断肝癌预后具有一定的临床价值。
Objective: To observe the expression of caspase-8 and caspase-9 in hepatocellular carcinoma and its clinicopathological significance. Methods: Fifty-three hepatocellular carcinoma patients from July 2012 to April 2013 in our hospital were selected as the observation group, and 50 normal liver tissues from the hospital during the same period were selected as the control group. In situ hybridization Two groups of patients Caspase-8 and Caspase-9 mRNA were detected. Results: The positive expression rate of caspase-8 and caspase-9 in the observation group and the control group were 84.91% (45/53), 88.68% (47/53) and 60.00% (30/50), 82.00% (41 / 50). The observation group and the control group all showed an increasing trend, the ratio was significantly different (P <0.05). Conclusion: The high expressions of caspase-8 and caspase-9 in hepatocellular carcinoma cells play an important role in the development and progression of hepatocellular carcinoma, and play a role in the proliferation and apoptosis of hepatoma cells. The expressions of caspase-8 and caspase-9 The expression of protein has certain clinical value for judging the prognosis of liver cancer.