用电激法将GFP基因成功转入分离的小麦 (TriticumaestivumL .)合子及早期原胚中。在电场强度 15 0V/cm、电容 2 5 μF、线性DNA浓度 2 0 0 μg/mL、电激缓冲液pH 7.2的条件下 ,得到GFP基因在早期原胚中的高频瞬间表达 (46 .7% )。与开花后 5d胚胎的最适电场强度相比 ,合子和早期原胚因较幼嫩而最适电场强度较低。电激后的一些早期原胚可以在KM8p培养基中培养并继续分裂 ,分裂后的细胞中也能观察到GFP基因的表达。此外 ,电激后的合子及 2细胞、4细胞和 8细胞原胚中没有看到基因表达的嵌合现象 The GFP gene was successfully electroporated into isolated wheat (Triticum aestivum L.) zygotes and early embryos. The transient expression of GFP gene in early embryo was observed under the conditions of electric field intensity of 15 0 V / cm, capacitance of 2.5 μF, linear DNA concentration of 200 μg / mL, and pH 7.2 of electroporation buffer (46.7 %). Compared with the optimal electric field intensity of embryos on the 5th day after flowering, the zygotic and early embryos were lower and the optimum electric field intensity was lower. Some early embryos after electroporation can be cultured in KM8p medium and continue to divide, and the expression of GFP gene can be observed in the cells after division. In addition, chimerism of gene expression was not seen in electroporated zygotes and in 2, 4 and 8-cell embryos