论文部分内容阅读
用~3H-TdR掺入艾氏腹水癌细胞DNA的方法,研究了几种抗癌药物的生化作用方式。癌细胞经与药物混合保温后,洗去药物,于不同时间加入~3H-TdR温育,测定其相对于对照组(用生理盐水代药物)的掺入率,发现药物对标记掺入的抑制大致可归纳为掺入递增及掺入递减两型曲线。经典的抗癌药物中:氮芥0时的掺入率为对照的65%,0.5小时下降到最低点(24%),1.2小时基本保持在0.5小时的水平,属递降型。氨甲喋呤450μg/ml在0小时掺入率为对照的50%,1小时上升接近对照组水平(90%),2小时呈掺入作用的回跃。阿糖
The method of ~ 3H-TdR incorporation into DNA of Ehrlich ascites cells was used to study the biochemical effects of several anticancer drugs. After the cancer cells were incubated with the drug and incubated, the drug was washed away and incubated with ~ 3H-TdR at different times. The incorporation rate of the drug into the control group (treated with physiological saline) was determined and the drug was found to inhibit the incorporation of the marker Can be roughly divided into the doping and doping into two types of curves. Classical anti-cancer drugs: nitrogen mustard at 0% incorporation of 65% of the control, 0.5 hours down to the lowest point (24%), 1.2 hours remained at 0.5 hours level, is a descending type. Methotrexate 450 [mu] g / ml incorporates 50% of the control at 0 hours, rises nearly 90% of control level at 1 hour, and is a spiking 2 h after incorporation. A sugar