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This study aimed to examine the differences in the morphological properties and proliferation of ol-factory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaf-folds and in two-dimensional culture on common flat culture plates.The proliferation rate of olfactory ensheathing cells in three-dimensional culture was higher than that in two-dimensional culture,as detected by an MTT assay.In addition,more than half of the olfactory ensheathing cells subcultured using the trypsinization method in three-dimensional culture displayed a spindly Schwann cell-like morphology with extremely long processes,while they showed a flat astrocyte-like morphology in two-dimensional culture.Moreover,spindle-shaped olfactory ensheathing cells tended to adopt an elongated bipolar morphology under both culture conditions.Experimental findings indicate that the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds are better than those in two-dimensional culture.
This study aimed to examine the differences in the morphological properties and proliferation of ol-factory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaf-folds and in two-dimensional culture on common flat culture plates. Proliferation rate of olfactory ensheathing cells in three-dimensional culture was higher than that in two-dimensional culture, as detected by an MTT assay. In addition, more than half of the olfactory ensheathing cells subcultured using the trypsinization method in three-dimensional culture displayed a spindly Schwann cell -like morphology with extremely long processes, while they showed a flat astrocyte-like morphology in two-dimensional culture. Moreover, spindle-shaped olfactory ensheathing cells tended to adopt an elongated bipolar morphology under both culture conditions. Experimental results indicate that the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collage n-heparan sulfate biological scaffolds are better than those in two-dimensional culture.