目的探讨癌基因ｃｙｃｌｉｎＤ１在肝癌发生发展中的作用以及与ＨＢＶ感染和临床病理之间的关系．方法取手术切除的肝细胞癌标本，用Ｓｏｕｔｈｅｒｎ杂交的方法对ｃｙｃｌｉｎＤ１和ＣＤＫ４癌基因以及ＨＢＶ的感染状态进行了检测，切片同时行病理检查．结果肝癌３９例中９例ｃｙｃｌｉｎＤ１存在２～３０倍的扩增（２３７％），其中１例检出重组杂交带；３７例中５例（１３５％）有ＣＤＫ４的扩增；ＨＢＶＤＮＡ在肝癌组织中检出的阳性率为９４７％，ＨＢＶＤＮＡ整合率为７４４％．相关分析发现，ｃｙｃｌｉｎＤ１的扩增与ＣＤＫ４扩增，ＨＢＶＤＮＡ整合，肿瘤的分化高低和肿块大小等之间有相关关系，相关系数ｒ分别为０５１，０３１，０３９和０５５，全部Ｐ＜００５．结论ｃｙｃｌｉｎＤ１的扩增与ＨＢＶＤＮＡ的整合密切相关，并在肝癌的恶化进展中起着重要作用． Objective To investigate the role of oncogene cyclinD1 in the development of hepatocellular carcinoma and its relationship with HBV infection and clinical pathology. Methods The specimens of surgically resected hepatocellular carcinoma were used to detect the cyclinD1 and CDK4 oncogenes and HBV infection status by Southern hybridization. Results Of the 39 cases of hepatocellular carcinoma, 9 cases of cyclinD1 were amplified from 2 to 30 times (23.7%). Among them, 1 case was detected with the recombinant hybrid band; 37 of 5 cases (13.5%) had the amplification of CDK4; HBV DNA The positive rate detected in HCC tissues was 94.7%, and the HBV DNA integration rate was 74.4%. Correlation analysis found that there was a correlation between the amplification of cyclinD1 and amplification of CDK4, integration of HBV DNA, tumor differentiation and tumor size, and the correlation coefficients were 051, 031, 039 and 055 respectively. , all P<005. Conclusion The amplification of cyclinD1 is closely related to the integration of HBV DNA and plays an important role in the progression of liver cancer.