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目的采用电化学法在粗化钛片表面制备纳米HA(Nano-HA)涂层并进行生物学评价。方法钛片表面粗化,采用电化学法在粗化钛片表面沉积Nano-HA涂层。通过场发射扫描电子显微镜(FSEM)、傅立叶红外光谱(FTIR)和X射线衍射仪(XRD)观察涂层晶体形貌、化学结构及组成;通过大鼠骨髓基质干(BMSCs)细胞培养验证该涂层的生物活性。结果 FSEM证实Nano-HA沉积在粗化钛片表面,直径约70～80nm,棒状晶粒截面为规则的六变形。FTIR和XRD分析表明该涂层为HA。与对照组相比,Nano-HA能够明显促进碱性磷酸梅(ALP)和骨钙素(OC)的表达。结论采用电化学法可在粗化纯钛表面制备Nano-HA涂层,并可促进BMSCs成骨分化。
Objective To prepare and evaluate the nano-HA coating on the surface of rough titanium by electrochemical method. Methods The surface of the titanium plate was roughened. Nano-HA coating was deposited on the surface of the roughened titanium plate by electrochemical method. The crystal morphology, chemical structure and composition of the coating were observed by field emission scanning electron microscopy (FSEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometer (XRD). The morphology and composition of the coating were observed by cell culture of bone marrow stromal cells (BMSCs) Layer biological activity. Results FSEM confirmed the deposition of Nano-HA on the surface of rough titanium, with a diameter of about 70-80 nm. The rod-like grain cross-section was regular hexagonal. FTIR and XRD analyzes showed that the coating was HA. Compared with the control group, Nano-HA can significantly promote the expression of alkaline phosphatase (ALP) and osteocalcin (OC). Conclusion Electro-chemical method can be used to prepare Nano-HA coating on rough surface of pure titanium and promote osteogenic differentiation of BMSCs.