论文部分内容阅读
用１段合成的１０８ｂｐＤＮＡ及免疫ＰＣＲ法，研究了ＳＬＥ血清抗双链ＤＮＡ抗体识别的ＤＮＡ序列。结果发现：１０个长度为１０８ｂｐ序列各异的已知ＤＮＡ片段，同具有亿万变化的１０８ｂｐＤＮＡ片段库一样，与抗双链ＤＮＡ抗体有良好反应，其中间２５个ｂｐ的变异不影响抗体与抗原反应；ＳＬＥ血清中抗体趋向于与易形成非ｂａｔａ－ＤＮＡ结构的ＤＮＡ特异结合
The DNA sequence recognized by anti-double-stranded DNA antibody of SLE serum was studied by using 108bp DNA synthesized in one step and immunoprecipitation method. The results showed that 10 known DNA fragments of 108bp in length had the same reactivity with anti-dsDNA antibody as those of billions of 108bp DNA fragments, and the 25 bp bp mutation did not affect the antibody and antigen Reaction; antibodies in SLE serum tend to specifically bind to DNA that readily forms non-bata-DNA structures